Yes, although this matters less than on other operating systems
On Android, most apps are written in Kotlin or Java, and are not specifically 32-bit or 64-bit. Developers build them from Kotlin or Java code, which are compiled into Dalvik bytecode for the Android Runtime. They upload their apps to app stores in that form, and devices download them in the same form.
Once downloaded, apps are compiled again, into machine code for the device. This machine code is either 32-bit or 64-bit, as suits the individual device. See "Glossary" below for the technical terms I've used.
This is quite different from Apple's iOS, iPadOS, and related operating systems. Apps for those devices are compiled into machine code by the developer, who uploads them in that form. This meant that Apple's transition from 32-bit to 64-bit was done earlier than Android's, and had less flexibility.
Native code in apps
Some Android apps, for various reasons, include machine code built by the developer, called "native code," rather like iOS apps. This gets uploaded to the app stores, downloaded again to devices, and run directly, without any compilation or translation steps along the way. An app that uses native code must provide separate versions for 32-bit and 64-bit devices if it is to be usable on both 32-bit and 64-bit devices.
Changes in Play Store rules
Since August 2021, apps that have native code, but only a 32-bit version of that code, have not been offered to 64-bit capable devices. This is presumably intended to push app developers who use native code to either (a) provide 64-bit native code or (b) stop using native code at all.
Changes in ARM processors
When 64-bit capable ARM processors first appeared, they could also run 32-bit code. However, that part is optional, and is gradually being dropped. Since 32-bit and 64-bit ARM native code are fairly different from each other (much more so than 32-bit and 64-bit x86), leaving out 32-bit makes processors simpler and thus cheaper to manufacture.
In the early days of 64-bit, there were some odd devices. There were ones that had 64-bit capable processors, but a 32-bit Android OS, and ones that had 64-bit processors and operating systems, but a 32-bit Android Runtime. However, those are now all in the past.
Nowadays, many of the newer ARM processors can't run 32-bit code. This is easiest to explain in terms of ARM Holdings' core designs, since those form the basis of many manufacturers' SoCs.
The early 64-bit cores could run 32-bit code happily, although the ability to run a 32-bit operating system was dropped at Cortex-A76. The ARMv9 cores that were introduced in starting in 2021 mostly can't run 32-bit code at all; the only exceptions are the Cortex-A710 and Cortex-A510.
That means, for example, that the Qualcomm Snapdragon 8 Gen 2 can only run 32-bit code on five of its eight cores, not including the fastest ones, and the Snapdragon 8 Gen 3 and later probably won't run 32-bit code at all. In the same way, the MediaTek Dimensity 9200 and later can only run 32-bit code on its slowest cores.
The first cores in each series from ARM that don't run 32-bit code are:
Glossary
Machine code
Computer programs consist of instructions telling the computer to do specific, simple things. The complicated and flexible behaviour of programs (which includes apps) is built out of many individual instructions. The instructions are encoded as numbers - because, to computers, everything is expressed as numbers - and the set of instructions that a computer can execute is its "machine code." The part of the computer which reads the machine code instructions and acts on them is the "processor" or "Central Processor Unit", abbreviated "CPU".
Different kinds of computer use different machine codes. Some manufacturers produce several different kinds of computers, with different machine codes. People have been designing machine codes for about 80 years (as of 2023) and new ideas are still being invented and put into use.
Most desktop computers use the "x86" or "x86-64" machine codes, developed by Intel Corporation and its competitor, Advanced Micro Devices, since the 1970s. Both those companies manufacture and sell CPUs. That's fine for desktop and laptop computers, which have space for lots of separate components.
Almost all mobile devices use the ARM family of machine codes, developed since the 1980s by ARM Holdings. ARM do not manufacture CPUs. Instead they license their designs to other companies, who manufacture "Systems-on-Chip" or "SoC"s that include the CPU and all the other electronics needed in a mobile device. Mobiles don't have space for lots of separate components: most of their small volume is taken up by the screen and the battery.
"Dalvik bytecode"?
"Bytecode" is a way of storing computer programs that isn't specific to a particular kind of computer. Many bytecodes exist, using different ways of representing programs. Dalvik bytecode was used in the Dalvik system for running Android apps that was used in Android versions before 5.0 "Lollipop" and was retained to provide compatibility with old apps.